AMaRONE investigates antimicrobial resistance (AMR), a growing global health concern where bacteria become resistant to antibiotics and infections become harder to treat. AMR is a One Health problem affecting humans and animals, with strong links to the environment, meaning that resistance genes can move across clinical, agricultural, and natural settings. The project uses publicly available long-read metagenomes from all One Health sectors to capture full-length resistance genes together with their mobile genetic elements and host genomes, allowing us to identify mobile latent antibiotic resistance genes and trace their movement from environmental reservoirs towards clinical contexts.

Building on this genomic foundation, AMaRONE verifies the phenotypic impact of selected resistance genes and develops proof-of-concept mitigation strategies that target their mobility and expression. The outcomes will support enhanced environmental and wastewater surveillance of AMR, contribute to risk prediction for emerging resistance threats, and inform proactive mitigation across One Health sectors.